Neuroprotective Effect of the Mixture of Gastrodiae elata and Glycyrrhizae uralensis In Vitro

Background: This study investigated the effect of a mixture Gastrodiae elata and Glycyrrhizae uralensis (GGW) on β-amyloid-induced neuronal damage in vitro. Methods: For finding appropriate GGW ratio, we performed MTT assays using ratios 5:5, 6:4, 7:3, 8:2 9:1 SK-N-SH cell SH-SY-5Y cell. Treatment with β-amyloid (10 μM) caused death overexpression acetylcholinesterase (ACHE) cells. treatment increased expression mitogen-activated protein kinases (MAPKs). In addition, detected antioxidant activity DCF-DA staining To determine oxidative stress, used hydrogen peroxidase-induced vitro model. Results: We selected 5:5 7:3 mixtures least cytotoxicity. The ratio (GGW73) decreased mRNA ACHE more than (GGW55). GGW73 suppressed p-ERK β-amyloid-treated Additionally, regulated reactive oxygen species (ROS) apoptotic factors β-amyloid-stimulated Conclusions: These results suggest that exerts neuroprotective anti-inflammatory effects also showed had protective against H2O2 induced an indicate potential as therapeutic agent for cognitive disorders.